《植物生理学报》 2017, 53(8): 1459-1467

油桐乙醛脱氢酶基因ALDH2B4和ALDH2B7的克隆与表达分析

刘美兰, 华园榕, 龙洪旭, 吕佳斌, 谭晓风^*

中南林业科技大学, 经济林培育与保护省部共建教育部重点实验室, 长沙410004

通信作者：谭晓风；E-mail: tanxiaofengcn@126.com

摘　要：

本研究运用反转录PCR (reverse transcription PCR, RT-PCR)技术, 从油桐(Vernicia fordii)近成熟种子中克隆了油桐乙醛脱氢酶基因家族两个成员的全长编码序列(CDS), 命名为VfALDH2B4和VfALDH2B7, NCBI登记号分别为MF179125和MF179126, CDS长度分别为1 617和1 626 bp, 分别编码538和541个氨基酸。通过蛋白结构分析发现这两个蛋白均含有乙醛脱氢酶基因家族特有的保守结构域, 且两个蛋白都与麻疯树(Jatropha curcas)的ALDH2B4和ALDH2B7亲缘关系最近。实时荧光定量PCR分析表明, 这两个基因在油桐各组织器官中均有表达, 其中VfALDH2B4在嫩叶的表达量最高, 是其他组织的21倍左右, 而fALDH2B7在花瓣的表达量最高, 是其他组织的62倍左右; 在油桐种子不同发育时期, 两个基因的表达也存在明显差异。此外, 两个基因表达还受高盐、干旱和脱落酸调控, 它们可能在油桐逆境胁迫应答和脱落酸信号途径中发挥作用。

关键词：油桐; 乙醛脱氢酶; 系统进化; 表达分析; 非生物胁迫

收稿：2017-01-06   修定：2017-07-02

资助：湖南省科技计划项目(2016NK2147)。

Cloning and expression analysis of Vernicia fordii aldehyde dehydrogenase genes ALDH2B4 and ALDH2B7

LIU Mei-Lan, HUA Yuan-Rong, LONG Hong-Xu, LÜ Jia-Bin, TAN Xiao-Feng^*

Central South University of Forestry and Technology / Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Changsha 410004, China

Corresponding author: TAN Xiao-Feng; E-mail: tanxiaofengcn@126.com

Abstract:

In this study, we reported the cloning and expression analysis of two aldehyde dehydrogenase (ALDH) family member genes in Vernicia fordii, named VfALDH2B4 and VfALDH2B7, respectively. The coding sequence (CDS) of ALDH2B4 gene is 1 617 bp, encoding 538 amino acids. The CDS of ALDH2B7 gene is 1 626 bp, encoding 541 amino acids. Protein structural analysis reveals that both proteins contain three conserved motifs in ALDH gene family, and both proteins have the closest relationship with JcALDH2B4 and JcALDH2B7, respectively. At the same time, we found both VfALDH2B4 and VfALDH2B7 expressed in tissues and organs by real-time PCR. In different developmental stages of V. fordii seed, both genes were significantly different in the expression. In addition, the expressions of these two genes were regulated by high-concentration salt, drought and abscisic acid treatments, which indicates that VfALDH2B4 and VfALDH2B7 genes may participate in stress response and abscisic acid pathway of V. fordii.

Key words: Vernicia fordii; aldehyde dehydrogenase; phylogeny; expression analysis; abiotic stress